AMX DESIGN XPRESS V 1.5 - PROGRAMMER GUIDE Instrukcja Użytkownika Pobierz pdf (Strona 6)

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December 16, 2004 3:54 pm, GSG_Preface.fm

Primer Express Version 3.0 Getting Started Guide vii

Preface

How to Use This Guide

Purpose of This

Guide

The Primer Express

Software Version 3.0 Getting Started Guide provides instructions

for automating the primer and probe design for Quantification and Allelic

Discrimination assays. It also explains how to manually annotate sequences and design

customized primer/prober sets.

Audience This guide is written for principal investigators and laboratory staff with general

knowledge of PCR and realtime-PCR terminologies and applications.

Assumptions This guide assumes that you have:

• A working knowledge of the assays

• Knowledge of primer and probe definitions

• Familiarity with Microsoft

Windows

XP operating systems

Text Conventions • Bold indicates user action. For example:

Type 0, then press Enter for each of the remaining fields.

• Italic text indicates new or important words and is also used for emphasis. For

example:

Before analyzing, always prepare fresh matrix.

• A right arrow bracket (>) separates successive commands you select from a drop-

down or shortcut menu. For example:

Select File > Open > Spot Set.

User Attention

Words

Two user attention words appear in Applied Biosystems user documentation. Each word

implies a particular level of observation or action as described below:

Note: Provides information that may be of interest or help but is not critical to the use of

the product.

IMPORTANT! Provides information that is necessary for proper instrument operation,

accurate chemistry kit use, safe use of a chemical, or proper software use.

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AMX DESIGN XPRESS V 1.5 - PROGRAMMER GUIDE Instrukcja Użytkownika Strona 6