AMX DESIGN XPRESS V 1.5 - PROGRAMMER GUIDE Instrukcja Użytkownika Pobierz pdf (Strona 16)

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December 16, 2004 3:54 pm, GSG_Chapter_4-02.fm

Chapter 2 Designing Primers and Probes for Quantification Assays

Automatically Designing Primers and Probes

Primer Express Software 3.0 Getting Started Guide 9

Notes

Automatically Designing Primers and Probes

This section describes automatically designing primers and probes for one sequence.

You can automatically design quantification primers and probes for multiple sequences

using the Batch Process Tool. For more information, see Primer Express Software

Version 3.0 Online Help.

Creating a

Quantification

Document

To create a new quantification document:

1. Select File > New. The New dialog box opens.

2. In the Type list, select Taq Man

MGB Quantification or TaqMan

Quantification.

3. Click OK.

The document window opens to the Sequence tab.

Loading a DNA

Sequence File

A sample sequence, NM_002217, is located in the sample sequences folder within the

Primer Express folder. You can use this sample file to experiment with the software and

design your primers and probes.

To load a sequence file:

1. Select Tools > Add DNA File ( ). Note you can also copy and paste or type

your sequence file in the Sequence tab.

2. At the Add DNA File dialog box, navigate to and select the desired file. For

information on the various file formats supported, see Primer Express Software

Version 3.0 Online Help.

3. Click Add. Primer Express software loads the nucleotide sequence from the file and

displays the sense strand in the Sequence tab (see “Figure 2. Sequence tab” on

page 10). The sequence serves as the starting point for primer and probe design.

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