AMX DESIGN XPRESS V 1.5 - PROGRAMMER GUIDE Instrukcja Użytkownika Strona 15

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DRAFT
December 16, 2004 3:54 pm, GSG_Chapter_4-02.fm
Chapter 2 Designing Primers and Probes for Quantification Assays
Overview
8 Primer Express Software 3.0 Getting Started Guide
Notes
Overview
About This
Chapter
This chapter provides information on using Primer Express Software Version 3.0 to
automatically design primers and probes for quantification assays (including SYBR
®
Green Dye Assays) using default parameters. It also includes information on how to
manually design primers and probes to obtain customized results.
Workflow
Figure 1. Quantification workflow
YesNo
Open or Create a
Quantification Document
Load a DNA Sequence File
Find Primers and Probes
View Results
Primers and Probes
Found?
Order Primers
and Probes
Manually Design
the Probe
Manually Design
the Primers
Desired
Primers and Probes
Found?
Edit Default
Parameters
Order Primers
and Probes
YesNo
Quantification Workflow
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